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- Title
Mid-infrared thin-film diamond waveguides combined with tunable quantum cascade lasers for analyzing the secondary structure of proteins.
- Authors
López‐Lorente, Ángela I.; Wang, Pei; Sieger, Markus; Vargas Catalan, Ernesto; Karlsson, Mikael; Nikolajeff, Fredrik; Österlund, Lars; Mizaikoff, Boris
- Abstract
Diamond has excellent optical properties including broadband transmissivity, low self-absorption, and a high refractive index, which have prompted its use for optical sensing applications. Thin-film diamond strip waveguides (DSWGs) combined with tunable quantum cascade lasers (tQCLs) providing an emission wavelength range of 5.78-6.35 μm (1735-1570 cm−1) have been used to obtain mid-infrared (MIR) spectra of proteins, thereby enabling the analysis of their secondary structure via the amide I band. Three different proteins were analyzed, namely bovine serum albumin (BSA), myoglobin, and γ-globulin. The secondary structure of BSA and myoglobin has a major contribution of α-helices, whereas γ-globulins are rich in β-sheet structures, which is reflected in the amide I band. A comparison of the spectra obtained via the combination of the tQCL and DSWG with spectra obtained using conventional Fourier transform infrared (FTIR) spectroscopy and a commercial diamond attenuated total reflection (ATR) element has been performed. It is shown that the main features evident in FTIR-ATR spectra are also obtained using tQCL-DSWG sensors.
- Subjects
THIN films; DIAMONDS; WAVEGUIDES; QUANTUM cascade lasers; INFRARED spectroscopy; PROTEIN structure
- Publication
Physica Status Solidi. A: Applications & Materials Science, 2016, Vol 213, Issue 8, p2117
- ISSN
1862-6300
- Publication type
Article
- DOI
10.1002/pssa.201600134