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- Title
IN VITRO PROPAGATION OF STAHLIANTHUS CAMPANULATUS KUNTZE, A RARE PLANT SPECIES FROM THAILAND.
- Authors
Mongkolsawat, W.; Saensouk, S.; Maknoi, C.; Saensouk, P.
- Abstract
A study was conducted to determine the effect of plant growth regulators on the initiation and growth of Stahlianthu campanulatus Kuntze, a rare plant species from Thailand. Rhizome buds derived from shoots were cut and cultured o modified MS medium supplemented with various concentrations of auxin, cytokinin and their combinations. Th cultures were kept under a temperature of 25±2 °C with fluorescent light for a 16 hours photoperiod/day for 12 week Multiple shoots cultured on MS medium supplemented with 4 mgL[sup -1] BAP, produced the highest number of shoots pe explant (5 shoots/explant). The highest lengths of shoots, 20.20, 20.06 and 20.12 cm, were obtained on the MS mediu supplemented with 4 mgL[sup -1] kinetin, 2 and 4 mgL[sup -1] BAP, respectively. The best rooting was observed in MS mediu supplemented with 1, 2 and 4 mgL[sup -1] kinetin, which produced 32.40-37.50 roots per shoot. MS medium supplemente with a combination of 4 mgL[sup -1] kinetin and 0.5 mgL[sup -1] IAA gave an average number of 3.0 shoots per explant with length of 21.28 cm. Acclimatization was successful when transplanted in soil and coconut fiber with 91.6% survivabilit potential.
- Subjects
PLANT micropropagation; CYTOKININS; ADENINE; FLUORESCENT lighting; ELECTRIC discharge lighting
- Publication
JAPS: Journal of Animal & Plant Sciences, 2018, Vol 28, Issue 2, p85
- ISSN
1018-7081
- Publication type
Article