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- Title
Helminth Colonization Is Associated with Increased Diversity of the Gut Microbiota.
- Authors
Lee, Soo Ching; Tang, Mei San; Lim, Yvonne A. L.; Choy, Seow Huey; Kurtz, Zachary D.; Cox, Laura M.; Gundra, Uma Mahesh; Cho, Ilseung; Bonneau, Richard; Blaser, Martin J.; Chua, Kek Heng; Loke, P'ng
- Abstract
Soil-transmitted helminths colonize more than 1.5 billion people worldwide, yet little is known about how they interact with bacterial communities in the gut microbiota. Differences in the gut microbiota between individuals living in developed and developing countries may be partly due to the presence of helminths, since they predominantly infect individuals from developing countries, such as the indigenous communities in Malaysia we examine in this work. We compared the composition and diversity of bacterial communities from the fecal microbiota of 51 people from two villages in Malaysia, of which 36 (70.6%) were infected by helminths. The 16S rRNA V4 region was sequenced at an average of nineteen thousand sequences per samples. Helminth-colonized individuals had greater species richness and number of observed OTUs with enrichment of Paraprevotellaceae, especially with Trichuris infection. We developed a new approach of combining centered log-ratio (clr) transformation for OTU relative abundances with sparse Partial Least Squares Discriminant Analysis (sPLS-DA) to enable more robust predictions of OTU interrelationships. These results suggest that helminths may have an impact on the diversity, bacterial community structure and function of the gut microbiota. Author Summary: Soil-transmitted helminths are carried by large numbers of people in developing countries. These parasites live in the gut and may interact with bacterial communities in the gut, also called the gut microbiota. To determine whether there are alterations to the gut microbiota that are associated with helminth infections, we examined the types of bacteria present in fecal samples from rural Malaysians, many of whom are helminth-positive and find it likely that helminth colonization alters the gut microbiota for rural Malaysians.
- Subjects
MALAYSIA; GUT microbiome; MICROBIAL communities; BACTERIAL communities; HELMINTHIASIS; DISCRIMINANT analysis
- Publication
PLoS Neglected Tropical Diseases, 2014, Vol 8, Issue 5, p1
- ISSN
1935-2727
- Publication type
Article
- DOI
10.1371/journal.pntd.0002880