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- Title
miR-22-3p Suppresses Adipocyte Formation of Telomerase Transformed Mesenchymal Stromal Cells (iMSC3) by Regulating Non-targeted MAPK Signaling Pathway.
- Authors
Alomran, Sara Sultan; Khattak, Muhammad Nasir Khan; Khan, Amir Ali; Abdallah, Sallam Hasan; Fayyad, Abeer Maher; Bajou, Khalid
- Abstract
Unraveling molecular mechanisms that govern adipocyte formation will lead to a greater understanding of obesity and subsequent treatment. In the current study, we assessed the effects of miR-22-3p, a non-coding RNA, on adipocyte differentiation from telomerase-transformed Mesenchymal Stromal Cells (iMSC3). The transfection of iMSC3 with miR-22-3p suppressed adipocyte differentiation as evident by reduction in lipid content and number of lipid droplets. While AKT3 is reported to be a target of miR-22-3p, our study indicated otherwise. Moreover, while miR-22-3p is known to target more than 500 genes, MAPK has not been identified as one of the more than 500 gene targets of miR-22-3p, though this pathway was upregulated in transfected iMSC3 that differentiated to adipocytes. Finally, miR-22-3p was observed to suppress adipocytes and was involved in adipocyte differentiation through non-targeted MAPK pathway upregulation rather than AKT3-related processes.
- Subjects
FAT cells; STROMAL cells; MITOGEN-activated protein kinases; ADIPOGENESIS; NON-coding RNA; TELOMERASE; TRANSFORMING growth factors; GENE targeting
- Publication
Pakistan Journal of Zoology, 2021, Vol 53, Issue 4, p1491
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/20191127131153