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- Title
Genetic Relationships Between Tribolium castaneum and T. confusum Based on Mitochondrial DNA Sequences.
- Authors
Qinglei Ming; Jiafei Shen; Chao Cheng; Zhaojun Feng
- Abstract
The flour beetles Tribolium castaneum (Herbst) and T. confusum (Jacquelin du Val) are economically important, morphologically similar and sympatrically distributed stored-product pests throughout the world. To clarify their genetic relationships, two regions of the mitochondrial DNA, the cytochrome c oxidase subunit I (COI) and the cytochrome b (Cytb), were sequenced for 100 individuals. Three and four haplotypes were identified for the COI and Cytb sequences, respectively, and there were no shared haplotypes between the two species in either sequence. Haplotype sequences within species differed by small numbers of nucleotide substitutions in the COI and Cytb region, and the substitutions did not result in the change of the encoded amino acids in both regions. In contrast, there were many substitutions between haplotypes of T. castaneum and T. confusum in the COI and Cytb regions, and the substitutions resulted in some encoded amino acid changes. Phylogeny incorporating two other Tribolium flour beetle species revealed that, the two species pairs, T. castaneum and T. freemani, and T. confusum and T. destructor, were more closely related than T. castaneum and T. confusum, which was not completely consistent with the phylogenetic relationships inferred from morphological data. Therefore, these results demonstrate that T. castaneum and T. confusum are genetically distinct from each other even though their morphology and size are very similar, and the COI and Cytb sequences are very useful for quantifying the genetic differentiation and phylogenetic relationships between species, especially morphologically similar species.
- Subjects
RED flour beetle; CONFUSED flour beetle; BEETLE genetics; INSECT morphology; MITOCHONDRIAL DNA; CYTOCHROME oxidase
- Publication
Pakistan Journal of Zoology, 2015, Vol 47, Issue 5, p1405
- ISSN
0030-9923
- Publication type
Article