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- Title
Cytotoxic and Genotoxic Effects of Arsenic on Primary Human Breast Cancer Cell Lines.
- Authors
Ahmad, Aftab; Ashraf, Sadia; Shakoori, A. R.
- Abstract
Breast cancer is one of the most common cancers, with high incidence rate among women Prevalence of breast cancer in Pakistan is highest in Asia. The present study was done to observe the effect of arsenic on breast cancer cell lines that was locally established Breast cancer tissues were taken from two hospitals and primary breast cancer cell lines were established by explants culture method. Neutral red based anti-proliferative assays were done to check the effect of arsenic on Pakistan Breast Cancer INMOL (PBCI) and Pakistan Breast Cancer Jinnali (PBCJ) cell lines. Comet assay was done to check the genotoxic effect of arsenic. Letal concentration 50 (LC50) for PBCI was 13 µ/ml on 24 h exposure and it shifted to 12 µ/ml when the cells were exposed for 48 h, while LC50, for PBCJ was 9 µ/ml. PBCJ cells proved to be more sensitive to arsenic than PBCI. When the number of cells were increased (1x104 cells per well) in 96 well plate LC50 for PBCI was 19 µ/ml. There was comet formation in arsenic treated samples compared to control. Ten different parameters were investigated for arsenic treated and control cells. The results indicated that arsenic had great cytotoxic and genotoxic effect on breast cancer cells and morphology of cells was totally changed with higher concentrations (12 µ/ml or higher) of arsenic.
- Subjects
PHYSIOLOGICAL effects of arsenic; BREAST cancer research; CANCER cells; GENETIC toxicology; CELL-mediated cytotoxicity; CELL lines
- Publication
Pakistan Journal of Zoology, 2015, Vol 47, Issue 1, p79
- ISSN
0030-9923
- Publication type
Article