We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Does etodolac affect TRPA1 functionality in vivo in human?
- Authors
Marynissen, Heleen; Mergaerts, Delphine; Bamps, Dorien; de Hoon, Jan
- Abstract
In preclinical research, etodolac, a non-steroidal anti-inflammatory drug, affected transient receptor potential ankyrin 1 (TRPA1) activation. Yet, whether the in vitro interaction between etodolac and TRPA1 translates to altered TRPA1 functionality in vivo in human remains to be investigated. A randomized, double-blinded, celecoxib-controlled study was conducted to assess the effect of etodolac on TRPA1-mediated dermal blood flow (DBF) changes on the forearm of 15 healthy, male volunteers aged between 18 and 45 years. Over four study visits, separated by at least five days wash-out, a single or four-fold dose of etodolac 200 mg or celecoxib 200 mg was administered orally. Two hours post-dose, TRPA1 functionality was evaluated by assessing cinnamaldehyde-induced DBF changes. DBF changes were quantified and expressed in Perfusion Units (PUs) using laser Doppler imaging during 60 min post-cinnamaldehyde application. The corresponding area under the curve (AUC0–60min) was calculated as summary measure. Statistical analysis was performed using Linear mixed models with post-hoc Dunnett. Neither the single dose of etodolac nor celecoxib inhibited the cinnamaldehyde-induced DBF changes compared to no treatment (AUC0–60min ± SEM of 17,751 ± 1,514 PUs*min and 17,532 ± 1,706 PUs*min vs. 19,274 ± 1,031 PUs*min, respectively, both p=1.00). Similarly, also a four-fold dose of both compounds failed to inhibit the cinnamaldehyde-induced DBF changes (19,235 ± 1,260 PUs*min and 19,367 ± 1,085 PUs*min vs. 19,274 ± 1,031 PUs*min, respectively, both p=1.00). Etodolac did not affect the cinnamaldehyde-induced DBF changes, suggesting that it does not alter TRPA1 functionality in vivo in human.
- Subjects
BIOMARKERS; CYCLOOXYGENASE 2; STATISTICS; FOREARM; IN vivo studies; PAIN measurement; PERIPHERAL neuropathy; NONSTEROIDAL anti-inflammatory agents; ORAL drug administration; PHOTOMETRY; BLOOD platelet aggregation; ETODOLAC; HEALTH status indicators; PERIPHERAL circulation; RANDOMIZED controlled trials; BLIND experiment; DESCRIPTIVE statistics; STATISTICAL sampling; PERFUSION imaging; RECEIVER operating characteristic curves; DATA analysis; CROSSOVER trials; DATA analysis software; STATISTICAL models; PERFUSION; PHARMACODYNAMICS
- Publication
Journal of Basic & Clinical Physiology & Pharmacology, 2023, Vol 34, Issue 4, p531
- ISSN
0792-6855
- Publication type
Article
- DOI
10.1515/jbcpp-2023-0004