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- Title
Detection of IgA Antibodies and Quantification of IgA Antibody--Producing Cells Specific for Ovalbumin or Trichinella spiralis in the Rat.
- Authors
van Loveren, H.; Osterhaus, A. D. M. E.; Nagel, J.; Schuurman, H. J.; Vos, J. G.
- Abstract
This report describes procedures to quantify IgA responses in the rat sensitized to ovalbumin or infected with the parasite Trichinella spiralis: an ELISPOT detecting specific IgA antibody-producing cell in lymph nudes, and an ELISA demonstrating IgA antibody in serum and gut mucosal scrapings. For this purpose a mouse monoconal anti-rat IgA antibody was produced. This IgGI-K1 antibody recognized rat IgA but not rat IgM, lgG, or IgE. It proved very suitable in both assays. Using this reagent we could demonstrate large numbers of IgA anti-ovalbumin-producing cells in the mesenteric lymph nodes 15 days alter sensitization to ovalbumin via the Peyer's patches. At 28 days after sensitization the numbers were much lower. IgA antibody titres to ovalbumin in serum were maximal between days 14 and 21 after immunization. Maximal numbers of IgA anti-T. spiralis-producing cells were found in the mesenteric lymph nodes 12 days after infection with muscle larvae, followed by a sharp decrease at 15 days. Maximal IgA anti-T. spiralis antibody titres in scrum and mucus scrapings of small intestines were found on days 10 and 12 after oral infection with the parasite.
- Subjects
IMMUNOGLOBULIN A; TRICHINELLA spiralis; LYMPH nodes; GASTROINTESTINAL system; ENZYME-linked immunosorbent assay; BLOOD plasma
- Publication
Scandinavian Journal of Immunology, 1988, Vol 28, Issue 3, p377
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1988.tb01463.x