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- Title
Autologous T lymphocytes recognize the tumour-derived immunoglobulin VH-CDR3 region in patients with B-cell chronic lymphocytic leukaemia.
- Authors
Rezvany, Mohammad Reza; Jeddi-Tehrani, Mahmood; Rabbani, Hodjattallah; Rudén, Ulla; Hammarström, Lennart; Österborg, Anders; Wigzell, Hans; Mellstedt, Håkan
- Abstract
We have previously shown that autologous T cells recognize leukaemic cells from patients with chronic lymphocytic leukaemia (B-CLL) in an MHC class I- and/or II-restricted manner. A candidate recognition structure might be the tumour cell-derived Ig VH complementarity-determining region (CDR)3. Three patients with B-CLL were analysed for the presence of autologous T cells recognizing the tumour-specific VH-CDR3 region. The VH region was shown to be mutated in all three patients. In two patients, a VH-CDR3-specific T-cell response was detected by proliferation assay, as well as by γ-interferon (IFN) production. The responses could be inhibited by monoclonal antibodies against MHC class II, but not MHC class I. In the third patient, a VH-CDR3 proliferative response was detected, which could be inhibited by an anti-MHC class I monoclonal antibody, but not by anti-MHC class II antibodies. No γ-IFN response could be detected in this patient. In no patient was an interleukin (IL)-4 response noted. Thus, in patients with B-CLL, naturally occurring T cells recognizing the tumour-unique VH-CDR3 region are present.
- Subjects
T cells; CHRONIC lymphocytic leukemia; INTERFERONS
- Publication
British Journal of Haematology, 2000, Vol 111, Issue 1, p230
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2000.02307.x