We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
T-Cell Activation in Patients With Irritable Bowel Syndrome.
- Authors
Öhman, Lena; Isaksson, Stefan; Lindmark, Ann-Charlotte; Posserud, Iris; Stotzer, P.-O.; Strid, Hans; Sjövall, Henrik; Simrén, Magnus
- Abstract
OBJECTIVES:Irritable bowel syndrome (IBS) has been found to be associated with low-grade immune activation in a subset of patients. We therefore investigated blood and colonic T-cell activity in IBS patients.METHODS:Blood samples were initially obtained from 74 IBS patients and 30 controls. Supplementary blood samples, to confirm data, were taken from another cohort (26 patients and 14 controls). In addition, colonic biopsies were taken from a third cohort (11 patients and 10 controls). Peripheral blood and colonic mononuclear cells were stimulated with anti-CD3/CD28 antibodies. Proliferation, cytokine secretion, and T-cell phenotype were investigated. IBS symptom severity was assessed.RESULTS:IBS patients displayed an activated phenotype with increased frequencies of blood T cells expressing CD69 and integrin β7/HLA-DR. Anti-CD3/CD28-stimulated blood and colonic T cells from IBS patients proliferated less than T cells from controls. IBS patients had an increased polyclonally stimulated T-cell secretion of IL-1β, which also weakly correlated with increased bowel habit dissatisfaction. Furthermore, despite normal frequencies of CD25high T cells in the blood of IBS patients, lower blood CD25high T-cell frequencies were modestly correlated with more bowel habit dissatisfaction and increased total IBS symptom severity.CONCLUSIONS:IBS patients have an increased frequency of activated T cells, demonstrated by the expression of activation markers and reduced proliferation in response to restimulation in vitro. The increased level of T-cell activation is consistent with the hypothesis of low-grade immune activation in IBS and may also be involved in symptom generation in IBS.Am J Gastroenterol 2009; 104:1205–1212; doi:10.1038/ajg.2009.116; published online 14 April 2009
- Subjects
T cells; IRRITABLE colon; PATIENTS; BLOOD; GENOTYPE-environment interaction; PHENOTYPES
- Publication
American Journal of Gastroenterology (Springer Nature), 2009, Vol 104, Issue 5, p1205
- ISSN
0002-9270
- Publication type
Article
- DOI
10.1038/ajg.2009.116