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- Title
Analysis of the Expression of CagA and Vac A and the Vacuolating Activity in 167 Isolates From Patients With Either Peptic Ulcers or Non-Ulcer Dyspepsia.
- Authors
Takata, Tohru; Fujimoto, Shuji; Anzai, Keizo; Shirotani, Takuro; Okada, Mitsuo; Sawae, Yoshiro; Ono, Junko
- Abstract
Objectives: The goals of this study were: 1) to examine the prevalence of cytotoxin-associated protein (CagA), vacuolating cytotoxin (VacA), and the vacuolating cytotoxin activity (VCA) in vitro of infecting <em>Helicobacter pylori</em> isolates and 2) to clarify the relation between the expression of these virulence factors and the occurrence of peptic ulceration. Methods: One hundred sixty-seven clinical isolates of <em>H. pylori</em> from patients with peptic ulcer disease (gastric ulcer, 62 eases; duodenal ulcer, 48 cases) and nonulcer dyspepsia (57 cases) were studied regarding their genetic and phenotypic properties. Results: Type 1 bacteria, which had both CagA and VCA, and type 2 bacteria, which did not express either CagA or VCA, represented 62.9% and 7.8%, respectively; the remaining 29.4% had an intermediate phenotype, expressing either CagA independent of the presence of VCA (CagA+VCA-) or vice versa (CagA-VCA+). CagA+VCA- and CagA-VCA+ bacteria represented 17.4% and 12.0%, respectively, both of which were more numerous than the type 2 category. The proportion of the CagA-positive isolates was significantly higher in both the duodenal ulcer (97.9%) and gastric ulcer (83.9%) patients than in the non-ulcer dyspepsia patients (61.4%) (<em>p</em> < 0.01). On the other hand, the proportion of VacA/VCA-positive isolates was not significantly different between peptic ulcer disease and nonulcer dyspepsia. Conclusions: The currently used classification of this bacterium based on the concomitant expression of CagA and VacA/VCA into the two major types is not adequate. The CagA-positive phenotype thus may be important as a virulence marker for peptic ulcer disease independent of the presence of VacA/VCA.
- Subjects
ANTINEOPLASTIC agents; PROTEINS; HELICOBACTER pylori; PEPTIC ulcer; INDIGESTION
- Publication
American Journal of Gastroenterology (Springer Nature), 1998, Vol 93, Issue 1, p30
- ISSN
0002-9270
- Publication type
Article
- DOI
10.1111/j.1572-0241.1998.030_c.x