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- Title
Effects of ketamine in methicillin-resistant Staphylococcus aureus and in silico interaction with sortase A.
- Authors
Coutinho, Tatiana do Nascimento Paiva; Barroso, Fátima Daiana Dias; da Silva, Cecília Rocha; da Silva, Anderson Ramos; Cabral, Vitória Pessoa de Farias; Sá, Lívia Gurgel do Amaral Valente; Cândido, Thiago Mesquita; da Silva, Lisandra Juvencio; Ferreira, Thais Lima; da Silva, Wildson Max Barbosa; Silva, Jacilene; Marinho, Emmanuel Silva; Cavalcanti, Bruno Coelho; Moraes, Manoel Odorico de; Nobre Júnior, Hélio Vitoriano; Andrade Neto, João Batista
- Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the main human pathogens and is responsible for many diseases, ranging from skin infections to more invasive infections. These infections are dangerous and expensive to treat because these strains are resistant to a large number of conventional antibiotics. Thus, the antibacterial effect of ketamine against MRSA strains, its mechanism of action, and in silico interaction with sortase A were evaluated. The antibacterial effect of ketamine was assessed using the broth microdilution method. Subsequently, the mechanism of action was assessed using flow cytometry and molecular docking assays with sortase A. Our results showed that ketamine has a significant antibacterial activity against MRSA strains in the range of 2.49–3.73 mM. Their mechanism of action involves alterations in membrane integrity and DNA damage, reducing cell viability, and inducing apoptosis. In addition, ketamine had an affinity for S. aureus sortase A. These results indicate that this compound can be used as an alternative to develop new strategies to combat infections caused by MRSA.
- Subjects
KETAMINE; METHICILLIN-resistant staphylococcus aureus; ANTIBACTERIAL agents; SKIN infections; DNA damage; MOLECULAR docking
- Publication
Canadian Journal of Microbiology, 2021, Vol 67, Issue 12, p885
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2021-0093