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- Title
Diverse culturable bacterial communities with cellulolytic potential revealed from pristine habitat in Indian trans-Himalaya.
- Authors
Thakur, Vikas; Kumar, Vijay; Kumar, Sanjay; Singh, Dharam
- Abstract
The Pangi–Chamba Himalaya (PCH) region is very pristine, unique, and virgin niche for bioresource exploration. In the current study, for the first time, the bacterial diversity of this region was investigated for potential cellulose degraders. A total of 454 pure bacterial isolates were obtained from diverse sites in the PCH region, and 111 isolates were further selected for 16S rDNA characterization based on ARDRA grouping. The identified bacteria belonged to 28 genera representing four phyla: Firmicutes, Proteobacteria, Actinobacteria, and Bacteroidetes. Pseudomonas was most abundant genus, followed by Bacillus, Geobacillus, Arthrobacter, Paenibacillus, and Flavobacterium. In addition, six putative novel bacteria (based on 16S rDNA sequence similarity) and thermophiles from non-thermogenic sites were also reported for the first time. Screening for cellulose degradation ability on carboxymethyl cellulose plates revealed that 70.92% of bacteria were cellulolytic. The current study reports diverse bacterial genera (Arthrobacter, Paenibacillus, Chryseobacterium, Pedobacter, Streptomyces, Agromyces, Flavobacterium, and Pseudomonas) with high capacity for cellulose hydrolysis and cellulolytic functionality at wide pH and temperature not previously reported in the literature. Diverse bacterial genera with high cellulolytic activity in broad pH and temperature range provide opportunity to develop a bioprocess for efficient pretreatment of lignocellulosic biomass, which is currently being investigated.
- Subjects
HIMALAYA Mountains; BACTERIAL communities; CELLULOLYTIC bacteria; BACTERIAL cultures; CELLULOSE; PSYCHROTROPHIC organisms
- Publication
Canadian Journal of Microbiology, 2018, Vol 64, Issue 11, p798
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2017-0754