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- Title
Purification and characterization of an exochitinase from Bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi.
- Authors
de la Vega, Luis Morales; Barboza-Corona, J. Eleazar; Aguilar-Uscanga, Maria G.; Ramírez-Lepe, Mario
- Abstract
A chitinolytic enzyme from Bacillus thuringiensis subsp. aizawai has been purified and its molecular mass was estimated ca. <NOBR>66 kDa</NOBR> by sodium dodecyl sulfate – polyacryamide gel electrophoresis (SDS–PAGE). The enzyme was able to hydrolyze chitin to chitobiosides but not carboxymethylcellulose, cellulose, pullulan, and laminarin. Optimal pH and temperature were detected at 6 and 50 °C, respectively. Stability, in the absence of substrate, was observed at temperatures less than 60 °C and pH between 5 and 8. Enzyme activity was significantly inhibited by K+ and EDTA and completely inhibited by Hg2+. Purified chitinase showed lytic activity against cell walls from six phytopathogenic fungi and inhibited the mycelial growth of both Fusarium sp. and Sclerotium rolfsii. The biocontrol efficacy of the enzyme was tested in the protection of bean seeds infested with six phytopathogenic fungi.
- Subjects
BACILLUS thuringiensis; PATHOGENIC fungi; ELECTROPHORESIS; ENZYMES; CHITIN; FUSARIUM; SCLEROTIUM rolfsii
- Publication
Canadian Journal of Microbiology, 2006, Vol 52, Issue 7, p651
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W06-019