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- Title
Effects of manipulating tetanic calcium on the curvature of the force‐velocity relationship in isolated rat soleus muscles.
- Authors
Kristensen, A. M.; Nielsen, O. B.; Overgaard, K.
- Abstract
Abstract: Aim: In dynamically contracting muscles, increased curvature of the force‐velocity relationship contributes to the loss of power during fatigue. It has been proposed that fatigue‐induced reduction in [Ca++]i causes this increased curvature. However, earlier studies on single fibres have been conducted at low temperatures. Here, we investigated the hypothesis that curvature is increased by reductions in tetanic [Ca++]i in isolated skeletal muscle at near‐physiological temperatures. Methods: Rat soleus muscles were stimulated at 60 Hz in standard Krebs‐Ringer buffer, and contraction force and velocity were measured. Tetanic [Ca++]i was in some experiments either lowered by addition of 10 μmol/L dantrolene or use of submaximal stimulation (30 Hz) or increased by addition of 2 mmol/L caffeine. Force‐velocity curves were constructed by fitting shortening velocity at different loading forces to the Hill equation. Curvature was determined as the ratio a/F0 with increased curvature reflecting decreased a/F0. Results: Compared to control levels, lowering tetanic [Ca++]i with dantrolene or reduced stimulation frequency decreased the curvature slightly as judged from increase in a/F0 of 13 ± 1% (<italic>P </italic>= < .001) and 20 ± 2% (<italic>P </italic>= < .001) respectively. In contrast, increasing tetanic [Ca++]i with caffeine increased the curvature (a/F0 decreased by 17 ± 1%; <italic>P </italic>= < .001). Conclusion: Contrary to our hypothesis, interventions that reduced tetanic [Ca++]i caused a decrease in curvature, while increasing tetanic [Ca++]i increased the curvature. These results reject a simple causal relation between [Ca++]i and curvature of the force‐velocity relation during fatigue.
- Subjects
LABORATORY rats; SOLEUS muscle; SKELETAL muscle; LOW temperatures; DANTROLENE
- Publication
Acta Physiologica, 2018, Vol 222, Issue 3, p1
- ISSN
1748-1708
- Publication type
Article
- DOI
10.1111/apha.12977