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- Title
Metabolic Interaction of the Active Constituents of Coptis chinensis in Human Liver Microsomes.
- Authors
Songcan Liu; Xinfeng Zhang; Furong Qiu; Ping Miao; Shujiao Shen; Leilei Zhu; Jin Zeng; Jian Jiang
- Abstract
Coptis chinensis is commonly used in traditional Chinese medicine. The study investigated metabolic interaction of the active constituents (berberine, coptisine, palmatine, and jatrorrhizine) of Coptis chinensis in human liver microsomes. After incubation of the four constituents of Coptis chinensis in HLMs, the metabolism of the four constituents was observed by HPLC. The in vitro inhibition experiment between the active constituents was conducted, and IC50 value was estimated. Coptisine exhibited inhibitions against the formation of the two metabolites of berberine with IC50 values of 6.5 and 8.3 µM, respectively. Palmatine and jatrorrhizine showed the weaker inhibitory effect on the formation of the metabolites of berberine. Berberine showed a weak inhibitory effect on the production of coptisine metabolite with an IC50 value of 115 µM and palmatine and jatrorrhizine had little inhibitory effect on the formation of coptisine metabolite. Berberine, coptisine, and jatrorrhizine showed no inhibitory effect on the generation of palmatine metabolite (IC50 > 200 µM). The findings suggested that there are different degrees of metabolic interaction between the four components. Coptisine showed the strongest inhibition toward berberine metabolism.
- Subjects
LIVER physiology; PHYTOTHERAPY; ENZYMES; ALTERNATIVE medicine; CELL physiology; CHROMATOGRAPHIC analysis; DYNAMICS; GENES; METABOLISM; RESEARCH funding; PHYSIOLOGY
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2015, p1
- ISSN
1741-427X
- Publication type
journal article
- DOI
10.1155/2015/802903