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- Title
ACE2-Inhibitory Effects of Bromelain and Ficin in Colon Cancer Cells.
- Authors
Pakbin, Babak; Dibazar, Shaghayegh Pishkhan; Allahyari, Samaneh; Shariatifar, Hanifeh; Brück, Wolfram Manuel; Farasat, Alireza
- Abstract
Background and Objectives: Bromelain and ficin are aqueous extracts from fruits of Ananas comosus and Ficus carcia plants, used widely for medical applications. Angiotensin-converting enzyme 2 (ACE2) is a homolog of ACE, degrading Ang II to angiotensin 1-7 and decreasing the cellular concentration of Ang II. Materials and Methods: In this study, we investigated the ACE2-inhibitory, antiproliferative, and apoptosis-inducing effects of ficin and bromelain on caco-2 cells. Results: We found that bromelain and ficin significantly reduced the viability of human colon cancer cells with IC50 value concentrations of 8.8 and 4.2 mg/mL for bromelain after 24 and 48 h treatments, and 8.8 and 4.2 mg/mL for ficin after 24 and 48 h treatments, respectively. The apoptosis of the caco-2 cell line treated with bromelain was 81.04% and 56.70%, observed after 24 and 48 h. Total apoptotic proportions in caco-2 cells treated with ficin after 24 and 48 h were 83.7% and 73.0%. An amount of 1.6 mg/mL of bromelain and ficin treatments on caco-2 cells after 24 h revealed a higher decrease than that of other concentrations in the expression of ACE2 protein. Conclusions: In conclusion, bromelain and ficin can dose-dependently decrease the expression of ACE2 protein in caco-2 cells.
- Subjects
BROMELIN; PINEAPPLE; COLON cancer; CANCER cells; ANGIOTENSIN converting enzyme; FICUS (Plants); CELL death
- Publication
Medicina (1010660X), 2023, Vol 59, Issue 2, p301
- ISSN
1010-660X
- Publication type
Article
- DOI
10.3390/medicina59020301