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- Title
Effective strategies for creating self-compatible B. rapa by introgression of mutated SRK and SCR/SP11 genes from B. napus.
- Authors
Zhang, Xueli; Heng, Shuangping; Xiao, Chunxiu; Liu, Cong; Song, Liping; Tang, Liguang; He, Congan; Wang, Bincai; Wang, Aihua; Gao, Changbin
- Abstract
This article discusses effective strategies for creating self-compatible Brassica rapa (Chinese cabbage) plants by introducing mutated SRK and SCR/SP11 genes from Brassica napus (rapeseed). Self-incompatibility (SI) is a common trait in Brassica vegetable crops, but it hinders the development and propagation of inbred lines. The manipulation of SI genes, such as SRK and SCR/SP11, can lead to the creation of self-compatible materials. The researchers used CRISPR/Cas9 technology to mutate the BnSRK-1 gene in B. napus and then employed distant hybridization to introduce the mutated gene into B. rapa, resulting in the generation of self-compatible materials. This research has important implications for improving the breeding process of Brassica vegetable crops.
- Subjects
GENETIC variation; OUTCROSSING (Biology); BOK choy; PLANT hybridization; MOLECULAR cloning; CHINESE cabbage
- Publication
Molecular Breeding, 2024, Vol 44, Issue 7, p1
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-024-01487-4