We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
<sup>13</sup>C SPE MAS measurement of ligand concentration in compressible chromatographic beads.
- Authors
Elwinger, Fredrik; Dvinskikh, Sergey V.; Furó, István
- Abstract
A method for measuring the ligand concentration in heterogeneous materials like chromatography media is described. In this method, 13C single pulse excitation magic angle spinning NMR experiment with broadband ¹H decoupling is used to determine the peak integrals for a butyl ligand in the spectrum of a dried chromatography medium. Within a carefully controlled protocol, those integrals compared with that of the internal reference compound dimethyl sulfone provide the required volume concentration with an accuracy of ca 2%. The effects of temperature, degree of hydration, and other experimental parameters are discussed.
- Subjects
LIGANDS (Chemistry); CHROMATOGRAPHIC analysis; DIMETHYL sulfone; SPIN excitations; HYDRATION; AGAROSE
- Publication
Magnetic Resonance in Chemistry, 2015, Vol 53, Issue 8, p572
- ISSN
0749-1581
- Publication type
Article
- DOI
10.1002/mrc.4257