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- Title
Biocompatible PHB Production from Bacillus Species Under Submerged and Solid-State Fermentation and Extraction Through Different Downstream Processing.
- Authors
Pati, Swayamsidha; Maity, Sudipta; Dash, Ankita; Jema, Soubhagyalaxmi; Mohapatra, Swati; Das, Surajit; Samantaray, Devi P.
- Abstract
Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) and solid-state fermentation (SSF). Under SmF and SSF, 0.60 g l−1 and 1.56 g l−1 of PHB with 0.497 g l−1 of yellow fluorescent pigment (YFP) was produced. Fourier transform infrared (FTIR) absorption bands at 1719–1720 cm−1 indicate the presence of C=O group of PHB. Nuclear magnetic resonance (NMR) exhibited the typical chemical shift patterns of PHB, and crystallinity was confirmed from X-ray diffraction (XRD). The melting temperature (Tm), degradation temperature (Td) and crystallinity (Xc) of extracted PHB were found to be 171 °C, 288 °C and 35%, respectively. FACS (Fluorescence-activated cell sorting) confirmed cytocompatibility of PHB at 400 µg ml−1 in mouse fibroblast line. Moreover, biodegradability and elevated cytocompatibility of the PHB produced through SSF make them highly potential biomaterials to be used as a drug delivery carrier in future.
- Subjects
SOLID-state fermentation; NUCLEAR magnetic resonance; BACILLUS (Bacteria); DRUG carriers; FOURIER transforms; X-ray diffraction
- Publication
Current Microbiology, 2020, Vol 77, Issue 7, p1203
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-020-01922-7