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- Title
Molecular identification of Trichuris vulpis and Trichuris suis isolated from different hosts.
- Authors
Cristina Cutillas; Manuel de Rojas; Concepción Ariza; José Ubeda; Diego Guevara
- Abstract
Abstract??Trichuris suiswas isolated from the cecum of two different hosts (Sus scrofa domestica?swine andSus scrofa scrofa?wild boar) andTrichuris vulpisfrom dogs in Sevilla, Spain. Genomic DNA was isolated and internal transcribed spacers (ITS)1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The sequence ofT. suisfrom both hosts was 1,396?bp in length while that ofT. vulpiswas 1,044?bp. ITS1 of both populations isolated ofT. suiswas 661 nucleotides in length, while the ITS2 was 534 nucleotides in length. Furthermore, the ITS1 ofT. vulpiswas 410 nucleotides in length, while the ITS2 was 433 nucleotides in length. One hundred fifty-four nucleotides were observed along the 5.8S gene ofT. suisandT. vulpis. Intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all the individuals assayed. Sequence analysis of the ITSs and the 5.8S gene has demonstrated no sequence differences betweenT. suisisolated from both hosts (S. scrofa domestica?swine andS. scrofa scrofa?wild boar). Nevertheless, clear differences were detected between the ITS1 and ITS2 ofT. suisandT. vulpis. Furthermore, a comparative molecular analysis between both species and the previously published ITS1-5.8S-ITS2 sequence data ofTrichuris ovis,Trichuris leporis,Trichuris muris,Trichuris arvicolae, andTrichuris skrjabiniwas carried out. A common homology zone was detected in the ITS1 sequence of all species of trichurids.
- Subjects
PARASITES; DNA; GENOMES; POLYMERASE chain reaction
- Publication
Parasitology Research, 2007, Vol 100, Issue 2, p383
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-006-0275-z