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- Title
Expression of anti-NbHK single-chain antibody in fusion with NSlmb enhances the resistance to Nosema bombycis in Sf9-III cells.
- Authors
Zhang, Renze; Zheng, Shiyi; Huang, Hongyun; Sun, Xi; Huang, Yukang; Wei, Junhong; Pan, Guoqing; Li, Chunfeng; Zhou, Zeyang
- Abstract
Nosema bombycis is a destructive and specific intracellular parasite of silkworm, which is extremely harmful to the silkworm industry. N. bombycis is considered as a quarantine pathogen of sericulture because of its long incubation period and horizontal and vertical transmission. Herein, two single-chain antibodies targeting N. bombycis hexokinase (NbHK) were cloned and expressed in fusion with the N-terminal of Slmb (a Drosophila melanogaster FBP), which contains the F-box domain. Western blotting demonstrated that Sf9-III cells expressed NSlmb–scFv-7A and NSlmb–scFv-6H, which recognized native NbHK. Subsequently, the NbHK was degraded by host ubiquitination system. When challenged with N. bombycis, the transfected Sf9-III cells exhibited better resistance relative to the controls, demonstrating that NbHK is a prospective target for parasite controls and this approach represents a potential solution for constructing N. bombycis-resistant Bombyx mori.
- Subjects
SILKWORMS; DROSOPHILA melanogaster; INTRACELLULAR pathogens; GLUCOKINASE; WESTERN immunoblotting
- Publication
Bulletin of Entomological Research, 2022, Vol 112, Issue 4, p502
- ISSN
0007-4853
- Publication type
Article
- DOI
10.1017/S0007485321001036