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- Title
Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus.
- Authors
Kazuhisa Ouhara; Hitoshi Komatsuzawa; Toshihisa Kawai; Hiromi Nishi; Tamaki Fujiwara; Yoshihiro Fujiue; Masao Kuwabara; Koji Sayama; Koji Hashimoto; Motoyuki Sugai
- Abstract
Objectives The susceptibility of clinical isolates of Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), to host-derived cationic antimicrobial peptides was investigated. Methods We examined the susceptibility of 190 clinical strains of methicillin-susceptible S. aureus (MSSA) and 304 strains of MRSA to two different classes of cationic antimicrobial peptides: LL-37 and human β-defensin-3 (hBD3). Out of the total 494 clinical strains, a random selection of 54 S. aureus strains was examined to establish the relationship between the net charge, or zeta potential, of each strain and its susceptibility to hBD3 or LL-37. To further confirm bacterial susceptibility to either hBD3 or LL-37, we concurrently measured: (i) percentage survival after in vitro bacterial exposure and (ii) MBCs for both MRSA and MSSA strains. Results Of the 54 randomly selected S. aureus strains, those MRSA strains resistant to LL-37 showed significantly higher zeta potentials than those susceptible to LL-37 (P S. aureus when compared with MSSA strains (P Conclusions Clinical strains of MRSA, but not MSSA, that demonstrated an increased net charge also showed elevated resistance to LL-37, but not to hBD3.
- Subjects
STAPHYLOCOCCUS; METHICILLIN resistance; STAPHYLOCOCCUS aureus infections; DRUG resistance in microorganisms
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2008, Vol 61, Issue 6, p1266
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkn106