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- Title
Anticoagulant activity of low-molecular-weight sulfated derivatives of galactomannan from Cyamopsis tetragonoloba (L.) seeds.
- Authors
Mestechkina, N. M.; Shcherbukhin, V. D.; Bannikova, G. E.; Varlamov, V. P.; Drozd, N. N.; Tolstenkov, A. S.; Makarov, V. A.; Tikhonov, V. E.
- Abstract
Galactomannan from seeds of Cyamopsis tetragonoloba (L.) Taub. (guar) was depolymerized using immobilized enzymatic preparation celloviridin. A set of fragments whose molecular weights varied from 12.6 to 245.6 kDa was obtained. Sulfated derivatives of components of all fractions were synthesized, in which the content of HSO groups was 48.05 ± 2.31%. All preparations exhibited anticoagulant activity, which was recorded in vitro in two tests—alla and aXa. The antithrombin activity (aIIa) was high (up to 65–87 U/mg) and did not depend on the molecular weight of a sulfated derivative; in the second test (aXa), the effect of molecular weight was observed. Biospecific electrophoresis allowed us to detect the ability of galactomannan sulfates to form complexes with protamine sulfate, a classic antidote to heparin.
- Subjects
GUAR; MOLECULAR weights; ELECTROPHORESIS; ANTITHROMBINS; HEPARIN; POLYSACCHARIDES
- Publication
Applied Biochemistry & Microbiology, 2007, Vol 43, Issue 6, p650
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S0003683807060142