We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Isolation of Cell Membranes from Saccharomyces cerevisiae for Evaluation of Their Protein Composition.
- Authors
Aliverdieva, D. A.; Malygin, A. G.; Lagutina, L. S.; Sholtz, K. F.
- Abstract
We describe a simple method for the isolation of membrane fractions from Saccharomyces cerevisiae yeasts containing a complex of plasma membranes and cell walls. The method is based on cell disruption on an INBI flow disintegrator. This device spares subcellular structures, which simplifies the isolation of cell membranes. The membrane fraction obtained by this method was suitable for studies of the protein composition of these structures by means of two-dimensional gel electrophoresis.
- Subjects
CELL membranes; SACCHAROMYCES cerevisiae; ELECTROPHORESIS; ELECTROCHEMISTRY; PHASE partition; BIOLOGICAL membranes
- Publication
Applied Biochemistry & Microbiology, 2004, Vol 40, Issue 3, p296
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1023/B:ABIM.0000025955.26547.04