We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enzymatic hydrolysis as a valorization strategy of bovine lungs: Optimization of process variables and study of antioxidant capacity.
- Authors
Martinez, F. G.; Ambrosi, V. A.; Rocha, G.; Sancho, A. M.; Szerman, N.
- Abstract
Background: The increase in meat production would also imply an increase in the generation of by‐products or edible offal. These by‐products can be used, due to their high protein content, as a substrate to obtain hydrolysates with functional activities. This study aimed to valorize bovine lungs by producing hydrolysates with antioxidant capacity. Results: A response surface methodology study was carried out using a central composite design in which factors were temperature (43.2–76.8°C), enzyme/substrate ratio [ES: 0.65–4.85% (w/w)] using Alcalase 2.4 L/Flavourzyme (1:1) and pH (5.8–9.2). The antioxidant capacity of the obtained BLH (bovine lung hydrolysates) was evaluated by the ABTS, DPPH, and FRAP methods. The maximum antioxidant capacity was achieved at 53.°C, ES ratio of 2.3% (w/w), and pH of 8.2. Under optimal hydrolysis conditions, the antioxidant capacity increased rapidly from the beginning of the reaction to 30 min, reaching a maximum at 120 min; then, it decreased until the end (180 min). The degree of hydrolysis increased as hydrolysis occurred up to a maximum of 45%. Molecular weight distribution of BLH evaluated by Tricine‐SDS‐PAGE and SEC‐FPLC showed that bovine lung proteins hydrolyzed for 120 min presented low molecular weight peptides (<5 kDa), which are absent in the non‐hydrolyzed sample. Conclusion: In this work, we optimized the variables for producing BLH with antioxidant capacity using commercial proteases in a short reaction time, making it a worthwhile strategy for the recovery of by‐products from the meat industry.
- Subjects
OXIDANT status; ANTIOXIDANTS; RESPONSE surfaces (Statistics); BOS; CATALYSTS; HYDROLYSIS; LUNGS
- Publication
JSFA Reports, 2023, Vol 3, Issue 4, p161
- ISSN
2573-5098
- Publication type
Article
- DOI
10.1002/jsf2.110