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- Title
表达 NDV HN 和 IBDV VP2 融合蛋白的重组减毒沙门菌的构建及其生物学特性分析.
- Authors
刘莎莎; 何雷; 余祖华; 李静; 郁川; 廖成水; 程相朝; 李银聚; 张春杰; 贾艳艳; 丁轲; 殷月兰
- Abstract
In order to construct the recombinant attenuated Salmonella which expresses Newcastle disease vims (NDV) HN and Infectious bursal disease virus (IBDV) YP2 fusion protein and analyze its biological characteristics, the recombinant vector pYA-HN-VP2 was constructed by inserting the fusion gene fragment HN-VP2 into prokaryotic expression vector pYA3493, and SL1344ΔcrpΔcyaΔasd (pYA-HN-VP2) was constructed by transform the recombinant vector pYA-HN-VP2 into attenuated Salmonella typhimurium SL1344ΔcrpΔcyaΔasd. The results of western blot showed that SL1344ΔcrpΔcyaΔasd (pYA-HN-VP2) could express and secrete 84 ku HN-VP2 fusion protein, which could react with specific positive sera against HN or VP2 protein, respectively. Biological characteristics results showed that recombination incapacitates the strain digesting ferment maltose, sorbitol and lactose, and the growth rate of the recombinant strain was slower than wt SL1344. LD50, of recombinant strain reduced at least 5 Logarithmic units compared with SL1344 and recombinant plasmid pYA-HN-YP2 could be stably inherited in the recombinant salmonella strain. These results provide new insights for triple oral vaccine candidate of ND. IBD and salmonellosis investigation.
- Publication
Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao, 2019, Vol 41, Issue 6, p567
- ISSN
1008-0589
- Publication type
Article
- DOI
10.3969/j.issn.1008-0589.201808028