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- Title
New technique for mouse oocyte injection via a modified holding pipette.
- Authors
Lyu, Q. F.; Deng, L.; Xue, S. G.; Cao, S. F.; Liu, X. Y.; Jin, W.; Wu, L. Q.; Kuang, Y. P.
- Abstract
To improve mouse oocyte survival from intracytoplasmic sperm injection, the sharp tip of the injection pipette has been modified to have a flat end. Here, for the same goal but for a more convenient manipulation, a sharp injection pipette was kept whereas the holding pipette was modified to have a trumpet-shaped opening, which allows deeper injection into the oocyte as it is held. Mouse oocyte injection with mouse and human spermatozoa was performed at 37°C. For the injection of mouse oocyte with mouse sperm head, a significantly higher survival rate (83%) was achieved by utilizing the modified holding pipette than the conventional one (21%; P < 0.001) and the fertilization rates were normal and comparable for both methods (82% versus 81%). A superior survival rate (82%) and acceptable normal fertilization rate (71%) were also achieved by utilizing the modified holding pipette for interspecies ICSI (injecting mouse oocyte with human spermatozoon). Taken together, by utilizing a holding pipette with a trumpet-shaped opening, acceptable rates of mouse oocyte survival and fertilization can be achieved using a sharp injection pipette under conditions usual for human oocyte injection.
- Subjects
EMBRYO implantation; PIPETTES; OVUM; FERTILIZATION (Biology); MICE
- Publication
Reproductive BioMedicine Online (Reproductive Healthcare Limited), 2010, Vol 21, Issue 5, p663
- ISSN
1472-6483
- Publication type
Article
- DOI
10.1016/j.rbmo.2010.07.004