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- Title
Proteome analysis of prostate cancer.
- Authors
Kuruma, H.; Egawa, S.; Oh-Ishi, M.; Kodera, Y.; Maeda, T.
- Abstract
In this paper, we briefly review cancer proteomics in general, with particular attention to our proteome analyses of prostate cancer. Our efforts include development of new tools and novel approaches to discovering proteins potentially useful as cancer diagnostic and/or prognostic biomarkers or as therapeutic targets. To this end, we analyzed prostate cancer proteomes using two-dimensional gel electrophoresis employing agarose gels for the initial isoelectric focusing step (agarose 2-DE), with mass spectrometry used for protein identification. Agarose 2-DE offers advantages over the more widely used immobilized pH gradient 2-DE for separating high molecular mass proteins (15-500?kDa), thereby increasing its power to detect changes in the cancer's high-molecular mass proteomes.Prostate Cancer and Prostatic Diseases (2005) 8, 14-21. doi:10.1038/sj.pcan.4500764 Published online 12 October 2004
- Subjects
PROSTATE cancer; GEL electrophoresis; BIOMARKERS; PROTEINS; MASS spectrometry
- Publication
Prostate Cancer & Prostatic Diseases, 2005, Vol 8, Issue 1, p14
- ISSN
1365-7852
- Publication type
Article
- DOI
10.1038/sj.pcan.4500764