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- Title
Transformation of Human Umbilical Vein Endothelial Cells Induced by the Tumor Microenvironment of Nephroblastoma Cells.
- Authors
Sang, Bo-tao; Wang, Chang-dong; Guo, Jia-qi; Wu, Xiang-mei
- Abstract
Nephroblastoma is one of the most common renal malignancies in children, and tumor angiogenesis increases the risk of metastasis and recurrence. This study investigated the effect of the tumor microenvironment on angiogenesis in nephroblastoma cell and its molecular mechanism. The indirect co-culture model of human umbilical vein endothelial cells (HUVECs) was established using a conditioned medium from the nephroblastoma cell line G401. Cell medium was collected to prepare tumor-conditioned medium (TCM), which was applied to HUVECs. Western blotting and immunofluorescence assays were used to analyze the expression of tumor endothelial marker 8 (TEM8) and the elements phosphatidylinositol 3-kinase (PI3K)/protein kinase B (PKB)/AKT signaling pathway at the protein level. In addition, the proliferation, invasion, migration, and tube formation of HUVECs after induction by TCM were analyzed by the Cell Counting Kit-8 (CCK-8), as well as by transwell invasion, wound-healing, and matrigel tube formation assays. Compared with that of the control group, the expression of TEM8, p-PI3K/PI3K, and p-AKT/AKT was increased, and proliferation, invasion, migration, and tubulogenesis were significantly enhanced in HUVECs induced by TCM. A PI3K pathway inhibitor (LY294002) weakened the canalization of HUVECs. These findings suggest that the nephroblastoma microenvironment can promote the transformation of endothelial cells (ECs) into tumor endothelial cells (TECs), significantly enhancing their proliferation, invasion, migration, and tubular formation, and this effect may be related to the PI3K/AKT signaling pathway.
- Subjects
ENDOTHELIAL cells; UMBILICAL veins; NEPHROBLASTOMA; TUMOR microenvironment; CELL transformation; PI3K/AKT pathway
- Publication
Journal of Evolutionary Biochemistry & Physiology, 2023, Vol 59, Issue 2, p411
- ISSN
0022-0930
- Publication type
Article
- DOI
10.1134/S0022093023020096