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- Title
afila, the origin and nature of a major innovation in the history of pea breeding.
- Authors
Tayeh, Nadim; Hofer, Julie M. I.; Aubert, Grégoire; Jacquin, Françoise; Turner, Lynda; Kreplak, Jonathan; Paajanen, Pirita; Le Signor, Christine; Dalmais, Marion; Pflieger, Stéphanie; Geffroy, Valérie; Ellis, Noel; Burstin, Judith
- Abstract
Summary: The afila (af) mutation causes the replacement of leaflets by a branched mass of tendrils in the compound leaves of pea – Pisum sativum L. This mutation was first described in 1953, and several reports of spontaneous af mutations and induced mutants with a similar phenotype exist. Despite widespread introgression into breeding material, the nature of af and the origin of the alleles used remain unknown.Here, we combine comparative genomics with reverse genetic approaches to elucidate the genetic determinants of af. We also investigate haplotype diversity using a set of AfAf and afaf cultivars and breeding lines and molecular markers linked to seven consecutive genes.Our results show that deletion of two tandemly arranged genes encoding Q‐type Cys(2)His(2) zinc finger transcription factors, PsPALM1a and PsPALM1b, is responsible for the af phenotype in pea. Eight haplotypes were identified in the af‐harbouring genomic region on chromosome 2. These haplotypes differ in the size of the deletion, covering more or less genes.Diversity at the af locus is valuable for crop improvement and sheds light on the history of pea breeding for improved standing ability. The results will be used to understand the function of PsPALM1a/b and to transfer the knowledge for innovation in related crops.
- Subjects
COMPARATIVE genomics; PHENOTYPES; CROP improvement; HAPLOTYPES; ZINC-finger proteins; PEAS
- Publication
New Phytologist, 2024, Vol 243, Issue 3, p1247
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.19800