We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Higher fungi are a rich source of l-amino acid oxidases.
- Authors
Žun, Gašper; Kos, Janko; Sabotič, Jerica
- Abstract
l-Amino acid oxidases (LAO) are widely distributed enzymes but those from snake venoms have been studied the most. We describe a method for in-gel detection of LAO activities based on HO detection by a horseradish peroxidase-coupled reaction using o-phenylenediamine. Complex substrates and single l-amino acids were used successfully for screening LAO activities in higher fungi using crude aqueous extracts of fruiting bodies of 22 basidiomycetes and 1 ascomycete. Half of these samples exhibited one to two bands of LAO activities with mostly broad substrate specificities and a variety of apparent molecular masses ranging from 25 to 200 kDa that were generally more active at pH 5.5 than at pH 8.0. Mushrooms are shown to be a rich source of LAOs that could find use in various medical and biotechnological applications.
- Subjects
FUNGI; AMINO acid oxidase; SNAKE venom; HORSERADISH peroxidase; MUSHROOMS
- Publication
3 Biotech, 2017, Vol 7, Issue 3, p1
- ISSN
2190-572X
- Publication type
Report
- DOI
10.1007/s13205-017-0813-7