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- Title
Histone deacetylase 9 interacts with SiHAT3.1 and SiHDA19 to repress dehydration responses through H3K9 deacetylation in foxtail millet.
- Authors
Kumar, Verandra; Singh, Babita; Singh, Roshan Kumar; Sharma, Namisha; Muthamilarasan, Mehanathan; Sawant, Samir V; Prasad, Manoj
- Abstract
Climate change inflicts several stresses on plants, of which dehydration stress severely affects growth and productivity. C4 plants possess better adaptability to dehydration stress; however, the role of epigenetic modifications underlying this trait is unclear. In particular, the molecular links between histone modifiers and their regulation remain elusive. In this study, genome-wide H3K9 acetylation (H3K9ac) enrichment using ChIP-sequencing was performed in two foxtail millet cultivars with contrasting dehydration tolerances (IC403579, cv. IC4—tolerant, and IC480117, cv. IC41—sensitive). It revealed that a histone deacetylase, SiHDA9 , was significantly up-regulated in the sensitive cultivar. Further characterization indicated that SiHDA9 interacts with SiHAT3.1 and SiHDA19 to form a repressor complex. SiHDA9 might be recruited through the SiHAT3.1 recognition sequence onto the upstream of dehydration-responsive genes to decrease H3K9 acetylation levels. The silencing of SiHDA9 resulted in the up-regulation of crucial genes, namely, SiRAB18 , SiRAP2.4, SiP5CS2 , SiRD22 , SiPIP1;4 , and SiLHCB2.3 , which imparted dehydration tolerance in the sensitive cultivar (IC41). Overall, the study provides mechanistic insights into SiHDA9-mediated regulation of dehydration stress response in foxtail millet.
- Subjects
FOXTAIL millet; DEHYDRATION; DEACETYLATION; SIRTUINS; HISTONE acetylation; ACETYLATION
- Publication
Journal of Experimental Botany, 2024, Vol 75, Issue 3, p1098
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erad425