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- Title
The Lymantria dispar IPLB-Ld652Y Cell Line Transcriptome Comprises Diverse Virus-Associated Transcripts.
- Authors
Sparks, Michael E.; Gundersen-Rindal, Dawn E.
- Abstract
The enhanced viral susceptibility of the gypsy moth (Lymantria dispar)-derived IPLB-Ld652Y cell line has made it a popular in vitro system for studying virus-related phenomena in the Lepidoptera. Using both single-pass EST sequencing and 454-based pyrosequencing, a transcriptomic library of 14,368 putatively unique transcripts (PUTs) was produced comprising 8,476,050 high-quality, informative bases. The gene content of the IPLB-Ld652Y transcriptome was broadly assessed via comparison with the NCBI non-redundant protein database, and more detailed functional annotation was inferred by comparison to the Swiss-Prot subset of UniProtKB. In addition to L. dispar cellular transcripts, a diverse array of both RNA and DNA virus-associated transcripts was identified within the dataset, suggestive of a high level of viral expression and activity in IPLB-Ld652Y cells. These sequence resources will provide a sound basis for developing testable experimental hypotheses by insect virologists, and suggest a number of avenues for potential research.
- Subjects
LEPIDOPTERA; LYMANTRIA dispar; INSECT viruses; CELL lines; GENETIC transcription; VIRAL cell transformation; NUCLEOTIDE sequence; VETERINARY virology
- Publication
Viruses (1999-4915), 2011, Vol 3, Issue 11, p2339
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v3112339