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- Title
Multi-omics studies of Nannochloropsis salina cultivated under suboptimal temperatures.
- Authors
GILL, SABA
- Abstract
American Heart Society recommends consumption of 1g Omega-3 fatty acids per day to prevent heart diseases. The oleaginous marine microalga Nannochloropsis salina has received attention for its high oil yield (37-60% wt.) and production of the dietary polyunsaturated fatty acids, ω-3 eicosapentaenoic acid (EPA). The incorporation of unsaturated fatty acids into membrane lipid pools is particularly important for maintaining membrane fluidity and cellular structure at suboptimal temperatures. We found that N. salina grown under "cold stress" (≥10°C below optimal temperature) results in increased desaturation of fatty acids. Here, we demonstrate the effects of cold stress under 16:8 hr day:night cycle on N. salina using four temperature regimes: 5°C, 10°C, 15°C and 25°C, where 25°C was used as control. Physiological stress was determined by measuring growth rate, chlorophyll fluorescence and dissolved oxygen measurements. Total lipids were measured using FAME analysis. Gene expression studies were carried out targeting the genes involved in Kennedy pathway for tri-acylglycerol (TAG) synthesis. We performed Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometry to study the variation in different lipid classes under sub optimal temperatures. Metabolomics was carried out using GC TOF MS, and the results were analyzed for statistical significance using MetaboAnalyst V3.0.
- Subjects
EFFECT of temperature on algae; ALGAL metabolites; ALGAL growth; UNSATURATED fatty acids; ION cyclotron resonance spectrometry; GENE expression; ALGAE
- Publication
New Mexico Journal of Science, 2016, Vol 50, Issue 1, p67
- ISSN
0270-3017
- Publication type
Article