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- Title
Extra-genital Neisseria gonorrhoeae infections with genetic mutations conferring ciprofloxacin resistance among men who have sex with men and transgender women in Lima, Peru.
- Authors
Qquellon, Jazmin; Vargas, Silver K; Eguiluz, Maria; Vasquez, Francesca; Durand, David; Allan-Blitz, Lao-Tzu; Konda, Kelika A; Ochoa, Theresa J; Caceres, Carlos F; Klausner, Jeffrey D
- Abstract
Background: The increasing prevalence of drug-resistant Neisseria gonorrhoeae (NG) infections has caused great concern. Ciprofloxacin remains the empiric antimicrobial recommended to treat NG infections in Peru disregarding the susceptibility profile of circulating NG strains. We report the prevalence of individuals infected with NG strains presenting mutations in the gyrA gene that confers ciprofloxacin resistance. Methods: We conducted a descriptive study assessing extragenital swab samples collected from a cohort of men who have sex with men and transgender women in Lima, Peru. Anal and pharyngeal NG positive swabs for Aptima Combo 2 assay (Hologic Inc., USA) were used for DNA extraction. We performed TaqMan real time PCR assays to detect a point mutation at codon Ser91 of the gyrase A (gyr A) gene. Results: From 156 individuals who had at least one positive sample for NG reported by the Aptima assay, 80 individuals had at least one amplified DNA for the gyrA gene. We found that 67 of them (84.0%) were infected with a gyrA -mutated NG strain at the Ser91 codon. Conclusions: We report a high prevalence of gyrA mutation conferring ciprofloxacin resistance among individuals with extragenital NG infection. Empirical treatment of NG needs to be urgently updated in Peru in concordance with international guidelines.
- Publication
International Journal of STD & AIDS, 2023, Vol 34, Issue 4, p245
- ISSN
0956-4624
- Publication type
Article
- DOI
10.1177/09564624221147326