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- Title
Polyhydroxy Sterols Isolated From Ganoderma sinense Spores and Their Cytotoxic Activities.
- Authors
Danhong Lian; Xin Zhong; Lian Li; Li Gu; Yimei Zheng; Xin Liu
- Abstract
Background: The fruiting body of Ganoderma sinense Zhao, Xu et Zhang iscertified as an authoritative medical material in the Chinese Pharmacopoeia 2020 edition and has been used as a crude drug for a long time. G sinense spores are crucial part of the fruiting body of G sinense and exhibited antitumor and immune-enhancing activities according to our previous reports. However, there were few studies about the chemical composition of G sinense spores. This study was aimed at the structure determination and antitumor effect of the sterols isolated from G sinense spores. Methods: Modern chromatographic methods were applied to isolate compounds from the ruptured spores of G sinense. Their chemical structures were elucidated by analyses of one-dimensional and two-dimensional ¹H nuclear magnetic resonance, and high-resolution electrospray ionization mass spectrometry data. The cytotoxicities of the isolated compounds against 3 tumor cell lines, human non-small cell lung cancer A549 and 95D cells, and human hepatocellular carcinoma HepG2 cells were measured by Cell Counting Kit-8. Results: A novel ergosterol derivative, (22E,24R)-3β,5β,6α,7α,14β-pentahydroxyergosta-8,22-dien-15-one (1), and 5 known sterols, (22E,24R)-3β,5α,9α,14β-tetrahydroxyergosta-7,22-dien-6-one (2), (22E,24R)-ergosta-7,9(11),22-triene-3β,5α,6β-triol (3), (22E,24R)-ergosta-7,9(11),22-triene-3β,5α,6β,14α- tetrol (4), β-daucosterine (5), and (22E,24R)-ergosta-7,22-diene-3β,5α,6β,9α-tetrol (6), were obtained from the ruptured spores of G sinense. Compound 1 exhibited cytotoxicity against A549 cells and HepG-2 cells with IC50 values of 65.12 ±4.76 and 97.34 ±6.36 μM, respectively. Compounds 1, 2, and 4 were obtained from G sinense for the first time. Conclusion: This paper is a continuation of an investigation of the chemical ingredients from G sinense spores and their antitumor effect. Only compound 1 showed weak cytotoxicities against A549 cells and HepG-2 cells.
- Subjects
ERGOSTEROL; ELECTROSPRAY ionization mass spectrometry; STEROLS; SPORES; GANODERMA; NON-small-cell lung carcinoma
- Publication
Natural Product Communications, 2024, Vol 19, Issue 1, p1
- ISSN
1934-578X
- Publication type
Article
- DOI
10.1177/1934578X231171513