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- Title
Convallatoxin Inhibits Cell Proliferation and Induces Cell Apoptosis by Attenuating the Akt-E2F1 Signaling Pathway in K562 Cells.
- Authors
Li, Man; Xu, Wangwang; Zhang, Lu; Gao, Erke; Han, Xiaojin; Chen, Zhengxu
- Abstract
Objective : To determine the effect of convallatoxin on K562 cell proliferation and apoptosis. Methods : CCK-8 assay was used to detect cell proliferation; PI staining, JC-1 staining, and Annexin V-FITC/PI double staining were used to analyze the cell cycle, cell mitochondrial membrane potential, and cell apoptosis; and Western blotting was used to detect cleaved caspase-9, cleaved caspase-3, Bcl-2, Bax, and E2F1 expression and Akt phosphorylation. Subsequently, AutoDock software was used to determine the interaction between convallatoxin and Akt1. Results : Upon treatment with convallatoxin, the proliferation of K562 cells was inhibited, the cells were arrested at the S and G2/M phases, and cell apoptosis was significantly induced. In addition, Akt phosphorylation and E2F1 expression were significantly decreased, whereas E2F1 overexpression rescued convallatoxin-induced cell proliferation and apoptosis. In addition, a molecular docking assay indicated that convallatoxin could bind to Akt1. Conclusion : Convallatoxin inhibited cell proliferation and induced mitochondrial-related apoptosis in K562 cells by reducing the Akt-E2F1 signaling pathway, indicating that it is a potential agent for treating leukemia.
- Subjects
INHIBITION of cellular proliferation; CELL proliferation; CELLULAR signal transduction; CELL cycle; APOPTOSIS; MEMBRANE potential
- Publication
Natural Product Communications, 2022, Vol 17, Issue 11, p1
- ISSN
1934-578X
- Publication type
Article
- DOI
10.1177/1934578X221136929