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- Title
The Involvement of Neural Retina Pax6 in Lens Fiber Differentiation.
- Authors
Reza, Hasan M.; Yasuda, Kunio
- Abstract
Proper eye formation depends on specific interactions between neural and ectodermal tissues coupled with temporally distinct gene expression and a regulated sequence of signaling events. The homeobox gene Pax6 is vitally important to the entire process of eye development in both vertebrates and invertebrates. Pax6 expression for the retina anlage has been shown to be indispensable in the development of various retinal cells. Here, we report that Pax6 expression in neural tissue plays an important role in lens development. Expression of a dominant-negative version of Pax6 isoform that lacks 5a-exon sequence in developing optic vesicles (OV) of chick embryos led to arrest of lens development at the lens vesicle stage as well as optic cup deformation. To gain insights into the molecular events underlying deformed lens formation, we examined the expression of several transcription factors in the lens of Pax6-negative-OV eye. Importantly, L-Maf was downregulated while c-Maf was found normal in deformed lens. We detected a downregulation of fibroblast growth factor (FGF8) in the neural tissue. Our in vivo experiments suggest that Pax6 in neural retina regulates FGF8 expression, which may maintain L-Maf expression in the lens to be essential for later lens fiber differentiation. Copyright © 2004 S. Karger AG, Basel
- Publication
Developmental Neuroscience, 2004, Vol 26, Issue 5/6, p318
- ISSN
0378-5866
- Publication type
Article
- DOI
10.1159/000082273