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- Title
Identification of Metabolic Pathways Influenced by the G-Protein Coupled Receptors GprB and GprD in <i>Aspergillus nidulans</i>
- Authors
de Souza, Wagner R.; Morais, Enyara Rezende; Krohn, Nadia Graciele; Savoldi, Marcela; Goldman, Maria Helena S.; Rodrigues, Fernando; Caldana, Camila; Semelka, Charles T.; Tikunov, Andrey P.; Macdonald, Jeffrey M.; Goldman, Gustavo Henrique
- Abstract
Heterotrimeric G-protein-mediated signaling pathways play a pivotal role in transmembrane signaling in eukaryotes. Our main aim was to identify signaling pathways regulated by A. nidulans GprB and GprD G-protein coupled receptors (GPCRs). When these two null mutant strains were compared to the wild-type strain, the ΔgprB mutant showed an increased protein kinase A (PKA) activity while growing in glucose 1% and during starvation. In contrast, the ΔgprD has a much lower PKA activity upon starvation. Transcriptomics and 1H NMR-based metabolomics were performed on two single null mutants grown on glucose. We noted modulation in the expression of 11 secondary metabolism gene clusters when the ΔgprB and ΔgprD mutant strains were grown in 1% glucose. Several members of the sterigmatocystin-aflatoxin gene cluster presented down-regulation in both mutant strains. The genes of the NR-PKS monodictyphenone biosynthesis cluster had overall increased mRNA accumulation in ΔgprB, while in the ΔgprD mutant strain the genes had decreased mRNA accumulation. Principal component analysis of the metabolomic data demonstrated that there was a significant metabolite shift in the ΔgprD strain. The 1H NMR analysis revealed significant expression of essential amino acids with elevated levels in the ΔgprD strain, compared to the wild-type and ΔgprB strains. With the results, we demonstrated the differential expression of a variety of genes related mainly to secondary metabolism, sexual development, stress signaling, and amino acid metabolism. We propose that the absence of GPCRs triggered stress responses at the genetic level. The data suggested an intimate relationship among different G-protein coupled receptors, fine-tune regulation of secondary and amino acid metabolisms, and fungal development.
- Subjects
G protein coupled receptors; ASPERGILLUS nidulans; FUNGAL proteins; PLANT cellular signal transduction; EUKARYOTES; PLANT mutation; FUNGAL genetics; GENE regulatory networks
- Publication
PLoS ONE, 2013, Vol 8, Issue 5, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0062088