We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Progressive Activation of CD127+1322 Recent Thymic Emigrants into Terminally Differentiated CD1272132+ T-Cells in HIV-1 Infection.
- Authors
Sasson, Sarah C.; Zaunders, John J.; Seddiki, Nabila; Bailey, Michelle; McBride, Kristin; Koelsch, Kersten K.; Merlin, Kate M.; Smith, Don E.; Cooper, David A.; Kelleher, Anthony D.
- Abstract
Aim: HIV infection is associated with distortion of T-cell homeostasis and the IL-7/IL7R axis. Progressive infection results in loss of CD127+1322 and gains in CD1272132+ CD4+ and CD8+ T-cells. We investigated the correlates of loss of CD127 from the T-cell surface to understand mechanisms underlying this homeostatic dysregulation. Methods: Peripheral and cord blood mononuclear cells (PBMCs; CBMC) from healthy volunteers and PBMC from patients with HIV infection were studied. CD127+1322, CD127+132+ and CD1272132+ T-cells were phenotyped by activation, differentiation, proliferation and survival markers. Cellular HIV-DNA content and signal-joint T-cell receptor excision circles (sjTRECs) were measured. Results: CD127+1322 T-cells were enriched for naïve cells while CD1272132+ T-cells were enriched for activated/terminally differentiated T-cells in CD4+ and CD8+ subsets in health and HIV infection. HIV was associated with increased proportions of activated/terminally differentiated CD1272132+ T-cells. In contrast to CD127+1322 T-cells, CD1272132+ T-cells were Ki- 67+Bcl-2low and contained increased levels of HIV-DNA. Naïve CD127+1322 T-cells contained a higher proportion of sjTRECs. Conclusion: The loss of CD127 from the T-cell surface in HIV infection is driven by activation of CD127+1322 recent thymic emigrants into CD1272132+ activated/terminally differentiated cells. This process likely results in an irreversible loss of CD127 and permanent distortion of T-cell homeostasis.
- Subjects
HIV infections; CD antigens; HOMEOSTASIS; T cells; INTERLEUKIN-7; CELL differentiation
- Publication
PLoS ONE, 2012, Vol 7, Issue 2, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0031148