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- Title
Molecular detection of Eastern Equine Encephalitis virus in mosquitoes from La Pintada (Antioquia).
- Authors
Hoyos L., Richard; Suaza V., Juan; Tenorio, Antonio; Uribe, Sandra; Gallego-Gómez, Juan
- Abstract
Objective. The detection of emerging and re-emerging arboviruses in mosquitoes from urban and rural areas, is fundamental for predict possible epidemic outbreaks in human populations. The Municipality of La Pintada (Antioquia), is characterized by the presence of dry tropical forest relicts, fishing, tourism, farms and mining. An entomological research was performed for explore the possible circulation of arboviruses of public health importance. Materials and methods. Mosquitoes were captured in urban and rural sites in February-April of 2012. The specimens were stored in liquid nitrogens tanks and were grouped using taxonomic keys for genera. RNA extraction from pools and generic/nested RT-PCR was performed for Flavivirus, Alphavirus, Orthobunyavirus (Group Bunyamwera) and Phlebovirus. Results. 1274 mosquitoes were collected, mainly belonging to Culex and Aedes genera. RNA extracts of 64 pools were tested by RT-PCR and one pool was positive for Alphavirus. Sequencing of the RTPCR product and the analysis with sequences storage in GenBank designate the presence of Eastern equine encephalitis virus (EEEV). Conclusions. This is the first record of natural infection from EEEV in mosquitoes from La Pintada (Antioquia), an area with ecological elements that favor the emergence of emerging and re-emerging arboviruses of medical and veterinarian importance.
- Subjects
EASTERN equine encephalomyelitis virus; ARBOVIRUSES; MOSQUITO vectors; EPIDEMICS; NUCLEIC acid isolation methods; REVERSE transcriptase polymerase chain reaction
- Publication
Revista MVZ Córdoba, 2015, Vol 20, Issue 3, p4800
- ISSN
0122-0268
- Publication type
Article
- DOI
10.21897/rmvz.49