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- Title
Combined DNA Analysis from Stool and Blood Samples Improves Tumor Tracking and Assessment of Clonal Heterogeneity in Localized Rectal Cancer Patients.
- Authors
Parigger, Thomas; Gassner, Franz Josef; Drothler, Stephan; Scherhäufl, Christian; Hödlmoser, Alexandra; Schultheis, Lena; Bakar, Aryunni Abu; Huemer, Florian; Greil, Richard; Geisberger, Roland; Weiss, Lukas; Zaborsky, Nadja
- Abstract
Objectives: In this study, stool samples were evaluated for tumor mutation analysis via a targeted next generation sequencing (NGS) approach in a small patient cohort suffering from localized rectal cancer. Introduction: Colorectal cancer (CRC) causes the second highest cancer-related death rate worldwide. Thus, improvements in disease assessment and monitoring that may facilitate treatment allocation and allow organ-sparing "watch-and-wait" treatment strategies are highly relevant for a significant number of CRC patients. Methods: Stool-based results were compared with mutation profiles derived from liquid biopsies and the gold standard procedure of tumor biopsy from the same patients. A workflow was established that enables the detection of de-novo tumor mutations in stool samples of CRC patients via ultra-sensitive cell-free tumor DNA target enrichment. Results: Notably, only a 19% overall concordance was found in mutational profiles across the compared sample specimens of stool, tumor, and liquid biopsies. Conclusion: Based on these results, the analysis of stool and liquid biopsy samples can provide important additional information on tumor heterogeneity and potentially on the assessment of minimal residual disease and clonal tumor evolution.
- Subjects
CIRCULATING tumor DNA; RECTAL cancer; DNA analysis; NUCLEOTIDE sequencing; CANCER patients
- Publication
Technology in Cancer Research & Treatment, 2024, p1
- ISSN
1533-0346
- Publication type
Article
- DOI
10.1177/15330338241252706