We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Enhancement of ε-poly- l-lysine (ε-PL) production by a novel producer Bacillus cereus using metabolic precursors and glucose feeding.
- Authors
Chheda, Anuj; Vernekar, Madhavi
- Abstract
Epsilon poly- l-lysine (ε-PL) is a homo-biopolymer with approximately 25-30 l-lysine residues. It is a promising natural biopolymer widely used in food and pharmaceutical industry. The present work reports enhanced production of ε-PL with a novel producer Bacillus cereus using amino acids and TCA cycle intermediates in the fermentation medium. Among the various amino acids and TCA cycle intermediates tested 2 mM l-aspartic acid and 5 mM citric acid gave ε-PL yield of 145.5 and 230 mg/L, respectively. A combination of citric acid after 24 h and l-aspartic acid after 36 h improved ε-PL yield from 85 mg/L (control) to 335 mg/L. Glucose feeding strategy along with metabolic precursors was employed which further enhanced ε-PL yield to 565 mg/L. Thus, more than sixfold increase in ε-PL yield was achieved suggesting the potential of Bacillus cereus as a novel ε-PL producer.
- Publication
3 Biotech, 2015, Vol 5, Issue 5, p839
- ISSN
2190-572X
- Publication type
Article
- DOI
10.1007/s13205-015-0291-8