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- Title
Blockade of IL-6 alleviates bone loss induced by modeled microgravity in mice.
- Authors
He, Baorong; Yin, Xinhua; Hao, Dingjun; Zhang, Xuefang; Zhang, Zhen; Zhang, Ke; Yang, Xiaobin
- Abstract
This study investigated the effects of blockade of IL-6 on bone loss induced by modeled microgravity (MG). Adult male mice were exposed to hind-limb suspension (HLS) and treated with IL-6-neutralizing antibody (IL-6 nAb) for 4 weeks. HLS in mice led to upregulation of IL-6 expression in both sera and femurs. IL-6 nAb treatment in HLS mice significantly alleviated bone loss, evidenced by increased bone mineral density of whole tibia, trabecular thickness and number, bone volume fraction of proximal tibiae, and ultimate load and stiffness of femoral diaphysis. IL-6 nAb treatment in HLS mice significantly enhanced levels of osteocalcin in sera and reduced levels of deoxypyridinoline. In MC3T3-E1 cells exposed to MG in vitro, IL-6 nAb treatment increased mRNA expression and activity of alkaline phosphatase, mRNA expression of osteopontin and runt-related transcription factor 2, and protein levels of osteoprotegerin and decreased protein levels of receptor activator of the NF-κB ligand. In RAW254.7 cells exposed to MG, IL-6 nAb treatment downregulated mRNA expression of cathepsin K and tartrate-resistant acid phosphatase (TRAP) and reduced numbers of TRAP-positive multinucleated osteoclasts. In conclusion, blockade of IL-6 alleviated the bone loss induced by MG.
- Subjects
BONES; RUNX proteins; BONE density; TERIPARATIDE; REDUCED gravity environments; ACID phosphatase; BONE shafts
- Publication
Canadian Journal of Physiology & Pharmacology, 2020, Vol 98, Issue 10, p678
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/cjpp-2019-0632