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- Title
Genetic Specificity of Primary and Secondary Proliferative and Cytotoxic Responses of Human Lymphocytes Grown in Continued Culture.
- Authors
Schendel, D. J.; Wank, R.; Bonnard, G. D.
- Abstract
Human T peripheral blood lymphocytes were grown in continued culture using conditioned medium obtained from phylohaemagglutinin-stimulated, pooled human leucocytes. These cultured T cells (CTC) were tested in mixed lymphocyte culture (MLC) and cell-mediated lympholysis (CML) assays lo determine the genetic specificity of their proliferative and cytotoxic responses. Primary responses were measured after initial in vitro stimulation by allogeneic cells. and secondary responses were measured after a second in vitro stimulation by allogeneic cells. Both primary and secondary proliferative responses were round lo be stimulated by alloantigens controlled by the HLA region and, more specifically, by antigens of the HLA-D region, in accordance with the responses of normal peripheral blood T lymphocytes. When CTC were established from unsensitized PBL and then stimulated with altogeneic cells, they could respond by proliferation in MLC but in contrast to PDL they did not show subsequent cylotoxic responses. On the other hand. CTC established from PBL that had been stimulated first with allogeneic cells in either primary or secondary MLC displayed high levels of cytotoxic reactivily In CML. The strongesi cytotoxicity was directed against allospecificilies controlled by the HLA region and specific for the MLC-stimulating cells, but lower levels of cross-reactive cytotoxicily were also observed.
- Subjects
LYMPHOCYTES; ANTIGENS; T cells; LEUCOCYTES; IMMUNOGLOBULINS; KILLER cells
- Publication
Scandinavian Journal of Immunology, 1980, Vol 11, Issue 1, p99
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00214.x