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- Title
Mitogenic Activation of Human Lymphocytes: a Protein A Plaque Assay Evaluation of Polyclonal B-Cell Activators.
- Authors
Hammarström, L.; Bird, A. G.; Smith, C. I. E.
- Abstract
Using the protein A plaque assay, the capacity of various polyclonal B cell activators to induce differentiation in human B lymphocytes was investigated. Dextran sulphate and native dextran were both virtually devoid of mitogenic properties. Lipupolysaccharide, however, was found to be a potent mitogen in human cells that, although giving rise to low DNA synthetic response. induced high numbers of immunoglobulin-synthesizing cells. Mean plaque-forming cell (PFC) numbers in healthy blood donors assayed on the optimal day (days 5-7) were 23.49.1 IgM/106 cells. 11.288 IgG;/106 cells, and 2643 IgA/106 cells. Values obtained in spleen cells, peaking at days4-6. were slightly higher. Purified protein derivative (PPD) was equally or even more effective than lipopolysaccharide (LPS) in generating PFC of different subclasses in peripheral blood with mean of 29,241 IgM/106, 21.269 IgG/106 and 3681 IgA/106. PPD furthermore induced a marked DNA synthetic response in human lymphocytes. Those data suggest that LPS and PPD may both be used as functional markers in human cells when analysing patients with a suspected immunodeficiency state. It is suggested that cultures should be assayed using the protein A plaque assay, thereby being able not only to investigate the individual immunoglobulin classes but also to avoid the possible hazards involved in measuring antigen-specific responses in patients whose prior immunization lo the antigen tested can never be totally excluded.
- Subjects
LYMPHOCYTES; DEXTRAN; GLUCOSE; NUCLEIC acids; IMMUNOGLOBULIN G; BLOOD donors
- Publication
Scandinavian Journal of Immunology, 1980, Vol 11, Issue 1, p1
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00202.x