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- Title
Grease matrix as a versatile carrier of proteins for serial crystallography.
- Authors
Sugahara, Michihiro; Mizohata, Eiichi; Nango, Eriko; Suzuki, Mamoru; Tanaka, Tomoyuki; Masuda, Tetsuya; Tanaka, Rie; Shimamura, Tatsuro; Tanaka, Yoshiki; Suno, Chiyo; Ihara, Kentaro; Pan, Dongqing; Kakinouchi, Keisuke; Sugiyama, Shigeru; Murata, Michio; Inoue, Tsuyoshi; Tono, Kensuke; Song, Changyong; Park, Jaehyun; Kameshima, Takashi
- Abstract
Serial femtosecond X-ray crystallography (SFX) has revolutionized atomic-resolution structural investigation by expanding applicability to micrometer-sized protein crystals, even at room temperature, and by enabling dynamics studies. However, reliable crystal-carrying media for SFX are lacking. Here we introduce a grease-matrix carrier for protein microcrystals and obtain the structures of lysozyme, glucose isomerase, thaumatin and fatty acid-binding protein type 3 under ambient conditions at a resolution of or finer than 2 Å.
- Subjects
PROTEIN crystallography; LUBRICATION &; lubricants; X-ray crystallography; LYSOZYME structure; GLUCOSE isomerase; THAUMATINS
- Publication
Nature Methods, 2015, Vol 12, Issue 1, p61
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.3172