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- Title
Robust, synergistic regulation of human gene expression using TALE activators.
- Authors
Maeder, Morgan L; Linder, Samantha J; Reyon, Deepak; Angstman, James F; Fu, Yanfang; Sander, Jeffry D; Joung, J Keith
- Abstract
Artificial activators designed using transcription activator-like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.
- Subjects
HUMAN genes; GENE expression; DNA-binding proteins; GENETIC regulation; NUCLEASE genetics
- Publication
Nature Methods, 2013, Vol 10, Issue 3, p243
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.2366