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- Title
Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA).
- Authors
Sander, Jeffry D.; Dahlborg, Elizabeth J.; Goodwin, Mathew J.; Cade, Lindsay; Feng Zhang; Cifuentes, Daniel; Curtin, Shaun J.; Blackburn, Jessica S.; Thibodeau-Beganny, Stacey; Yiping Qi; Pierick, Christopher J.; Hoffman, Ellen; Maeder, Morgan L.; Khayter, Cyd; Reyon, Deepak; Dobbs, Drena; Langenau, David M.; Stupar, Robert M.; Giraldez, Antonio J.; Voytas, Daniel F.
- Abstract
Engineered zinc-finger nucleases (ZFNs) enable targeted genome modification. Here we describe context-dependent assembly (CoDA), a platform for engineering ZFNs using only standard cloning techniques or custom DNA synthesis. Using CoDA-generated ZFNs, we rapidly altered 20 genes in Danio rerio, Arabidopsis thaliana and Glycine max. The simplicity and efficacy of CoDA will enable broad adoption of ZFN technology and make possible large-scale projects focused on multigene pathways or genome-wide alterations.
- Subjects
ZINC-finger proteins; GENETIC engineering research; GENOMICS; ZEBRA danio; NUCLEASES; DNA
- Publication
Nature Methods, 2011, Vol 8, Issue 1, p67
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1542